Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture.

The circadian pacemaker circuit orchestrates rhythmic behavioral and physiological outputs coordinated with environmental cues, such as day/night cycles. The molecular clock within each pacemaker neuron generates circadian rhythms in gene expression, which underlie the rhythmic neuronal functions essential to the operation of the circuit. Investigation of the properties of the individual molecular oscillators in different subclasses of pacemaker neurons and their interaction with neuronal signaling yields a better understanding of the circadian pacemaker circuit. Here, we present a time-lapse fluorescent microscopy approach developed to monitor the molecular clockwork in clock neurons of cultured Drosophila larval brain. This method allows the multi-day recording of the rhythms of genetically encoded fluorescent circadian reporters at single-cell resolution. This setup can be combined with pharmacological manipulations to closely analyze real-time response of the molecular clock to various compounds. Beyond circadian rhythms, this multipurpose method in combination with powerful Drosophila genetic techniques offers the possibility to study diverse neuronal or molecular processes in live brain tissue.


Nature News & Views: 2017 in research

An article reporting results from our Department is one of the 7 ‘News & Views’ selected from this year by the editors of the prestigious journal Nature.


The mBRAVE Platform: The Next Generation of HTS Analysis

23.02.2018 14:00, 4059 (Sciences III)

Sujeevan Ratnasingham (Center for Biodiversity Genomics, University Guelph, Canada).
hosted by: Jan Pawlowski.


Our department hosts 12 research laboratories gathering close to 200 scientists, engineers and technical staff. Research topics cover a large variety of topics, such as developmental genetics and neurogenetics, regeneration, evo-devo, physics of biology, phylogenetics or anthropology.



Teaching life sciences at the University of Geneva is an important duty for all staff scientists. In addition to the bachelor programme, we also propose specific masters and PhD specialisations through various programmes.



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