Synergistic recognition of an epigenetic DNA element by Pleiohomeotic and a Polycomb core complex.

  • publication
  • 04-08-2005

Mohd-Sarip A, Cléard F, Mishra RK, Karch F, Verrijzer CP. Genes Dev. 2005 Aug;19(15):1755-60. 19/15/1755. 10.1101/gad.347005. PMC1182336.

Polycomb response elements (PREs) are cis-acting DNA elements that mediate epigenetic gene silencing by Polycomb group (PcG) proteins. Here, we report that Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to PREs. We identified a conserved sequence motif, named PCC-binding element (PBE), which is required for PcG silencing in vivo. PHO sites and PBEs function as an integrated DNA platform for the synergistic assembly of a repressive PHO/PCC complex. We termed this nucleoprotein complex silenceosome to reflect that the molecular principles underpinning its assemblage are surprisingly similar to those that make an enhanceosome.

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