2x genomes--depth does matter.

  • publication
  • 11-02-2010

Milinkovitch MC, Helaers R, Depiereux E, Tzika AC, Gabaldón T. Genome Biol. 2010 ;11(2):R16. gb-2010-11-2-r16. 10.1186/gb-2010-11-2-r16. PMC2872876.

Given the availability of full genome sequences, mapping gene gains, duplications, and losses during evolution should theoretically be straightforward. However, this endeavor suffers from overemphasis on detecting conserved genome features, which in turn has led to sequencing multiple eutherian genomes with low coverage rather than fewer genomes with high-coverage and more even distribution in the phylogeny. Although limitations associated with analysis of low coverage genomes are recognized, they have not been quantified.

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