Crystallization and preliminary crystallographic analysis of the importin-beta-SREBP-2 complex.

  • publication
  • 23-09-2003

Lee SJ, Sekimoto T, Yamashita E, Nagoshi E, Nakagawa A, Tanaka H, Yoneda Y, Tsukihara T. Acta Crystallogr. D Biol. Crystallogr. 2003 Oct;59(Pt 10):1866-8. S0907444903018328.

The nuclear-transport protein importin-beta mediates the nuclear import of the transcription factor SREBP-2 without requiring adaptor proteins such as importin-alpha. An importin-beta-SREBP-2 HLHZ domain complex was purified and crystallized. The crystals belong to space group P2(1)2(1)2(1) and show diffraction to at least 3.0 A resolution. The unit-cell parameters are a = 101.0, b = 113.2, c = 240.0 A. Structure determination using the MAD or SAD method is under way.

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