Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo.

  • publication
  • 28-03-2018

Zakany J, Duboule D. BMC Res Notes 2018 Mar;11(1):193. 10.1186/s13104-018-3307-8. 10.1186/s13104-018-3307-8. PMC5870235.

We had previously reported a mouse line carrying the Atypical female courtship (HoxD) allele, where an ectopic accumulation of Hoxd10 transcripts was observed in a sparse population of cells in the adult isocortex, as a result of a partial deletion of the HoxD gene cluster. Female mice carrying this allele displayed an exacerbated paracopulatory behavior, culminating in a severe mutilation of the studs' external genitals. To unequivocally demonstrate that this intriguing phenotype was indeed caused by an illegitimate function of the HOXD10 protein, we use CRISPR/Cas9 technology to induce a microdeletion into the homeobox of the Hoxd10 gene in cis with the HoxDallele.

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