Isolation, DNA Extraction, Amplification, and Gel Electrophoresis of Single-Celled Nonmarine Foraminifera (Rhizaria)

• publication
• 20-03-2024

Maria Holzmann. In: Amaresan, N., Chandarana, K.A. (eds) Practical Handbook on Soil Protists. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3750-0_31

Molecular tools are an important part to study the phylogeny and taxonomy of foraminifera. In the present chapter, isolation and extraction of these single-celled organisms will be described. Furthermore, the amplification of the 18S barcoding fragment in foraminifera and detection of amplified products by agarose gel electrophoresis will be detailed.

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