Isolation, DNA Extraction, Amplification, and Gel Electrophoresis of Single-Celled Nonmarine Foraminifera (Rhizaria)

  • publication
  • 20-03-2024

Maria Holzmann. In: Amaresan, N., Chandarana, K.A. (eds) Practical Handbook on Soil Protists. Springer Protocols Handbooks. Humana, New York, NY.

Molecular tools are an important part to study the phylogeny and taxonomy of foraminifera. In the present chapter, isolation and extraction of these single-celled organisms will be described. Furthermore, the amplification of the 18S barcoding fragment in foraminifera and detection of amplified products by agarose gel electrophoresis will be detailed.

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