Efficient mutation identification in zebrafish by microarray capturing and next generation sequencing

  • publication
  • 10-02-2011

Bontems, F., Baerlocher, L., Mehenni, S., Bahechar, I., Farinelli, L., & Dosch, R.. Biochemical and biophysical research communications, 405(3), 373–376. https://doi.org/10.1016/j.bbrc.2011.01.024

Fish models like medaka, stickleback or zebrafish provide a valuable resource to study vertebrate genes. However, finding genetic variants e.g. mutations in the genome is still arduous. Here we used a combination of microarray capturing and next generation sequencing to identify the affected gene in the mozartkugelp11cv (mzlp11cv) mutant zebrafish. We discovered a 31-bp deletion in macf1 demonstrating the potential of this technique to efficiently isolate mutations in a vertebrate genome.

see on Pubmed