Publications

Small leisure boat harbours have important aesthetic and recreational values in any country with a coastline. In Sweden, there are about 860 000 leisure boats, which is one of the world's highest numbers in relation to the country's population. However, small boat harbours also present a wide range of environmental problems, including the introduction of alien species and high pollution. In this study, we investigated the ecological quality status (EcoQS) of the Hinsholmskilen small boat harbour, located southwest of the city of Gothenburg (Sweden). We performed a reconnaissance survey of the harbour's previously unstudied benthic foraminiferal communities and analysed surface sediment (0–2 cm) samples for potentially toxic elements: copper (Cu), zinc (Zn), lead (Pb), cobalt (Co), nickel (Ni), chromium (Cr), mercury (Hg), and arsenic (As). The results show that, based on the total benthic foraminiferal distribution (dead and live specimens), the assemblages in Hinsholmskilen harbour represent a typical European estuarine community with highly abundant Ammonia and Elphidium species. Based on molecular and morphological data, we report the presence of two alien and putatively invasive species likely originating from Asia: Trochammina hadai and Ammonia confertitesta (phylotype T6). Both species have recently been identified elsewhere on the Swedish west coast based on molecular and morphological data but do not have a well-known distribution. The sediment analysis for potentially toxic elements showed that the harbour has good to high EcoQS corresponding to no or little deviation from reference conditions for Cd, Co, Ni, and Pb distribution. Some of the contaminants (Pb, As, Zn, and Cr) showed poor to bad EcoQS in the innermost harbour in proximity to high-pressure cleaning plants, where boats are usually lifted, cleaned, and prepared for winter storage on land. Finally, Cu and Hg showed consistently bad and poor EcoQS all over the harbour, reflecting the use of both metals as biocides in antifouling boat paints.

The role of circadian clocks in regulating metabolic processes is well known; however, their impact on metabolic states across species and life stages remains largely unexplored. This study investigates the relationship between circadian rhythms and metabolic regulation in the Drosophila larval fat body, a metabolic hub analogous to the mammalian liver and adipose tissue. Surprisingly, the fat body of period null mutants, which lack a functional circadian clock in all tissues, exhibited 12-hour rhythms in gene expression, particularly those involved in peroxisome function, lipid metabolism, and oxidative stress response. These transcriptomic rhythms were aligned with 12-hour oscillations in peroxisome biogenesis and activity, reactive oxygen species levels, and lipid peroxidation. Furthermore, period mutants exhibited 12-hour rhythms in body fat storage, ultimately leading to a net reduction in body fat levels. Collectively, our results identify clock-independent ultradian rhythms in lipid metabolism that are essential for larval survival and development.

Large numbers of spherical, organic-walled, monothalamous foraminifera were observed and isolated from the fine-sized sediment fraction collected at hadal depths in the Aleutian trench. The foraminifer was one of the few living species found among diatom frustules that make up most of this sediment fraction. Morphologically, the species resembles the genus Bathyallogromia described from the Weddell Sea and subsequently found in the abyssal Arctic Ocean, and other high latitude areas but never in such massive abundance. Molecular phylogenetic analyses based on partial 18S rRNA gene sequences confirmed its generic identification as a new species within the Bathyallogromia clade that is described here as Bathyallogromia brandtae sp.nov. Our study offers new evidence that deep-sea monothalamous foraminifera can proliferate in extreme hadal settings and have biogeographic distributions that are more restricted than is commonly assumed.

Neorotalia leeuwinensis n. sp. is morphologically and genetically distinct from other calcarinids that are present in very shallow waters along the western margin of Australia and has not been recognised amongst known species elsewhere. The distributions of calcarinids along the margin has been surveyed from 22 sites. Four distinct latitudinally-based regions are defined related to temperature. Neorotalia leeuwinensis n. sp. has a known latitudinal range of 30°S to 34°S (>19°C, 30-year average winter minimum sea temperature in the north and >18°C in the south). The main microhabitat observed for the species is the articulate coralline algae Amphiroa gracilis, which usually lives among a diversity of macrophytes in very shallow high-energy environments on the rock platforms. Test shape, robust peripheral spines, and pore tubes are related to the high-energy epibiont lifestyle on the algal branches. Significant marine and terrestrial climatic gradients along the west coastline of Australia from the tropics to the south apparently control calcarinid species distributions, but the reasons for endemism require much more study of environmental parameters and molecular analyses of species.

Blood vessels expand and contract actively as they continuously experience dynamic external stresses from blood flow. The mechanical response of the vessel wall is that of a composite material: its mechanical properties depend on its cellular components, which change dynamically as the cells respond to external stress. Mapping the relationship between these underlying cellular processes and emergent tissue mechanics is an ongoing challenge, particularly in endothelial cells. Here we assess the mechanics and cellular dynamics of an endothelial tube using a microstretcher that mimics the native environment of blood vessels. The characterization of the instantaneous monolayer elasticity reveals a strain-stiffening, actin-dependent and substrate-responsive behaviour. After a physiological pressure increase, the tissue displays a fluid-like expansion, with the reorientation of cell shape and actin fibres. We introduce a mechanical model that considers the actin fibres as a network in the nematic phase and couples their dynamics with active and elastic fibre tension. The model accurately describes the response to the pressure of endothelial tubes.

Many examples of self-organized embryonic patterning can be attributed to chemically mediated systems comprising interacting morphogens. However, mechanical patterning also contributes to the emergence of biological forms. For example, various studies have demonstrated that diverse patterns arise from elastic instabilities associated with the constrained growth of soft materials, which generate wrinkles, creases and folds. Here, we show that between days 12 and 13 of development, transient experimentally increased activity of the sonic hedgehog pathway in the chicken embryo, through a single intravenous injection of smoothened agonist (SAG), abolishes the Turing-like chemical patterning of reticulate scales on the ventral footpad and promotes a transition to mechanical labyrinthine skin folding. Using hybridization, nanoindentation and labelling of proliferating cells, we confirm that skin surface folding is associated with the loss of signalling placode pre-patterning as well as increased epidermal growth and stiffness. Using additional hydrocortisone treatments, we also demonstrate that experimentally induced hyper-keratinization of the skin mechanically restricts SAG-induced folding. Finally, we verify our experimental findings with mechanical growth simulations built from volumetric light sheet fluorescence microscopy data. Overall, we reveal that pharmacological perturbation of the underlying gene regulatory network can abolish chemical skin appendage patterning and replace it with self-organized mechanical folding.

Regional and tissue-wide regulation of signaling pathways orchestrates cellular proliferation and differentiation during organ development. In this study, we established an imaging platform for longitudinal analysis of liver development in live developing zebrafish. We generated hepatocyte-specific transgenic lines for kinase translocation reporters of extracellular signal-regulated kinase (Erk) and c-Jun N-terminal kinase (Jnk) signaling, and with these we captured signaling dynamics that govern rapid expansion of hepatocytes toward creation of the functioning liver at single-cell resolution. Our findings reveal Erk signaling fluctuations as the liver develops and introduce methodology for investigating cell-type specific signaling dynamics during organ morphogenesis.

In this study, we describe a novel monothalamous Foraminifera, discovered in shoreline sediment samples from the Southern Ocean. The individuals, approximately 75 μm in diameter, are relatively small for Foraminifera, mostly spherical, with an organic-walled test. Notably, these Foraminifera exhibit a unique behavior in culture: they surround themselves in planktonic diatoms, enabling them to float in the water column. This floating behavior is unusual for Foraminifera, which are often larger and possess a thick test made of calcite or agglutinated particles. We hypothesize that the small size of the organism, its lightweight organic test, and the habit of surrounding itself with centric diatoms may enable the floating behavior observed in culture and potentially aid dispersal in nature. Phylogenetic analysis of the 18S rDNA barcoding fragment places this undescribed organism as an independent lineage among monothalamid Foraminifera. We erect the novel genus Pensilisphaera with its type species Pensilisphaera antarcticaensis.

Benthic foraminifera are one of the major groups of eukaryotes living at cold seeps on the Arctic seafloor. However, their distribution and endemicity in these habitats have been largely debated. It is still unclear whether foraminiferal species commonly found in cold seeps differ genetically from those in deep-sea environments, and to what extent the seep community is distinct. To address these questions, we analyzed sediment DNA metabarcoding data specifically targeting foraminifera in different deep-water cold seep microhabitats (microbial mats, siboglinid tubeworms field) and reference sites within and outside the seep. Our results revealed microhabitat specificity among benthic foraminifera species. Microbial mats were dominated by a unique type of rDNA sequences assigned to a new lineage of monothalamid (single-chambered) foraminifera not previously reported from any other Arctic location. Other foraminiferal species were found across both seeps and reference stations. This study shows the presence of an endemic benthic foraminiferal species at cold seeps and confirms the existence of many common opportunistic species.

Snakes exhibit a broad variety of adaptive colors and color patterns, generated by the spatial arrangement of chromatophores, but little is known of the mechanisms responsible for these spectacular traits. Here, we investigate a mono-locus trait with two recessive alleles, motley and stripe, that both cause pattern aberrations in the corn snake.